Rat Skin Main Neutral Protease: Purification and Properties
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چکیده
منابع مشابه
Purification and properties of an acidic protein from rat skin.
An acidic protein, extractable in neutral salt solutions from rat skin, was markedly enriched when precipitated by dialysis against 0.5 M acetic acid. After dissolving the precipitate in 0.5 M Tris-HCl buffer, pH 8.0, the protein was disaggregated by the addition of the nonionic detergent Triton X-100 and purified by chromatography on Sephadex G-100 and DEAE-Sephadex A-50 columns. The protein i...
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Penicilliurn expamum grown in a medium with rice husk as a carbon source produced an extracellular protease. The protease enzyme was isolated from culture broth by fractionation with acetone and column chromatography on Sephadex G- 100 and DEAE A-50. The protease enzyme was purified about 17.47 fold, with a recovery of 14%. The purified protease was homogenous on SDS polyacrylarnide disc ge...
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ژورنال
عنوان ژورنال: Journal of Investigative Dermatology
سال: 1978
ISSN: 0022-202X
DOI: 10.1111/1523-1747.ep12541221